| Brantford TF Club | Bytown Storm Triathlon |
| Durham Dragons Athletics | ENSS |
| Equipe Endurance | Kalenjins |
| KINGSTON ACHILLES | Kingston TFC |
| Kingston Track & Field Club | kjn |
| KRRA | Laurel Creek T.F.C |
| Les Coureurs De Bois | Marie Rivier Dragons |
| Ottawa Lions | Petawawa Bell |
| R2R | Running Free/Markham Sole Sisters |
| Toronto Elite Raw Dawgz | Toronto Olympic Track & Field Club |
| Track East | Unnattached |